Risk Breakdown Structure - Deploying Genomic Surveillance
RBS sources:
https://www.biotechniques.com/coronavirus-news/opinion_where-are-the-sars-cov-2-genomes-from-east-africa/
h/t https://twitter.com/laura_boykin/status/1264200579720474624
https://twitter.com/kcorazo/status/1266322732288839680
They have the equipment and training.
But there is lack of collaboration (eg, sampling org to sequencing org).
Gov't employee mindset: needs top-down orders to change work direction
Lack of funding from govt (I suppose this is for reagents and other consumables considering they have equipment and training)
lab-in-suitcase papers
https://www.reddit.com/r/bioinformatics/comments/gk91mp/what_are_the_drawbacks_of_oxford_nanopore
- People tend to equate "cheap" with "easy". It's a very different process from other sequencing, even ignoring that labs tend to get sequencing done as a service rather than doing it themselves. Nanopore offer training, but it's very expensive, so most people try to muddle through themselves (hence the tendency for sequencers to collect dust).
- Oxford Nanopore's official sample prep protocols are not properly versioned, and have lots of traps for naive users (especially users who aren't used to working within a sequencing service facility). ONT also haven't said explicitly that it's okay to copy protocols into a proper protocol versioning service (e.g. protocols.io(https://protocols.io)), so there are various dances involved in what gets reported in research papers
- Software for working with nanopore reads is very new, tends to be command-line based, and hence is not very user-friendly.