Quick et al, 2016
Quick J, Loman NJ, Duraffour S, et al. Real-time, portable genome sequencing for Ebola surveillance. Nature. 2016;530(7589):228-232. doi:10.1038/nature16996 https://gyazo.com/647645d963d591012809db8bdc8e2349
Quick et al (2016) demonstrated that it is possible to pack a genomic surveillance laboratory in a suitcase and transport it to the field for on-site virus sequencing, generating results within 24 hours of sample collection. They used the Oxford Nanopore MinION for real-time genomic surveillance of the Ebola virus epidemic in the field in Guinea. This was the first study that investigated the usage of the MinION for a viral outbreak. They present the advantages of using the MinION, how it works, and the tradeoffs of its usage. The authors also presented their innovations that allowed this operation. For instance, they designed a laboratory protocol to permit EBOV genome sequencing on the MinION that employed a targeted reverse transcriptase polymerase chain reaction (RT-PCR) in order to isolate sufficient DNA for sequencing. This protocol was adapted for rabies virus (RABV) in Brunker et al, 2020. The same protocol is also the basis for the one used in Meredith LW, Hamilton WL, Warne B, et al., 2020, adapted for SARS-CoV-2. This SARS-CoV-2 sequencing protocol is also the one proposed in this paper (see Methods 3.2). Quick et al also lists down the equipment and reagents needed to follow the protocol. This list serves as the basis for the Bill of Materials in this project (see Appendix 3).