がんゲノム診療における細胞検体の取扱い指針

第 1.0 版　202106

https://gyazo.com/70600e1c95540a9424b2f6a079ee416a

1. 「がんゲノム診療における細胞検体の取扱い指針」発刊にあたり........................... 5

2. ゲノム診療における細胞検体利用への期待................................................................. 7

3. 細胞検体の適切な取扱い.............................................................................................. 11

3.1 検体処理前の取扱い............................................................................................... 13

3.2 液状化検体細胞診（LBC）検体............................................................................ 15

3.2.1 LBC 検体作製.................................................................................................. 15

3.2.2 腫瘍細胞含有割合の評価 ............................................................................... 15

3.2.3 核酸抽出法 ...................................................................................................... 15

3.2.4 その他留意点................................................................................................... 16

3.3 ホルマリン固定パラフィン包埋（FFPE）セルブロック検体............................ 19

3.3.1 セルブロック作製........................................................................................... 19

3.3.2 腫瘍細胞含有割合の評価 ............................................................................... 19

3.3.3 核酸抽出法 ...................................................................................................... 20

3.3.4 その他留意点................................................................................................... 20

3.4 塗抹標本.................................................................................................................. 23

3.4.1 既染標本の取扱い........................................................................................... 23

3.4.2 未染標本の取扱い........................................................................................... 23

4. 細胞診材料の取扱いに関する実証データ................................................................... 25

5. 参考資料 ........................................................................................................................ 29

6. 補遺 ............................................................................................................................... 33

7. 調査資料........................................................................................................................ 39