Zero Blunt Topo Cloning
#2_Molecular
Aim
Materials
Manual
PCR product
Methods
table:TOPO mixture
PCR product 2 μL
Salt (x6) 0.5 μl
Topo vector (x6) 0.5 μL
Total 3 μL
Room temperature for 5 min or 16 ℃ for 30 min
Transform with DH5a competent cells.
(DH5a is sensitive to CcdB gene, but JM109 is not)
Be careful which vectors are going to be used, Km or Amp resistant vectors.
Remarks
Date :2025/4/17
Modified Date :2025/4/17 y-goto.icon
Author :ao.icon