Frozen stock of S.pombe
Aim
To make the S.pombe strain stock.
Materials
YE + 20% Glycerol
0.5% Yeast Extract
3% glucose
20% Glycerol in DDW and autoclaved
ampicillin might inhibit contamination (option) 100 ug/ml
Methods
Prepare the fresh cell
Streak on the plate a day ago (1/4 ~1/8 10cm dish is sufficient). or YEA culture up to 1.0*10^7 cells/ml (1~5ml).
Dispense 100~1000ul YE+20% Glycerol into 1.5ml tube.
Suspend cell pellet ~10^9 cells/ml
Freeze and store at -80℃ deep freezer.
Frozen stock is scratched by blue tip or spoon and streak onto the fresh plate.
* Direct liquid culture from frozen stock tend to be contaminated.
Remarks
y-goto.icon
Date :20170706
Modified Date :
Author : Yuhei Goto