Crystal violet staining for colony formation cells
Aim
To stain live cells by crystal violet
Materials
0.005 % Crystal violet (50% MetOH+50%PBS) stock solution
Dissolve crystal violet powder in H2O and then add methanol. No sterilization procedures are required. Store solution in the dark at room temperature. Use within 2 mo.
Methods
Cells were washed once with PBS and then applyed 10x diluted 0.005 % Crystal violet (50% MetOH+50%PBS) stock solution in PBS at R.T for 1 h
rinse off crystal violet with PBS and then count colony numbers
Remarks
DON'T discard Crystal violet staining solution. Keep this solution into specific container.
Date :2019.04.18
Modified Date :2020.12
Author :Reina Itoh
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