Antibiotics for mammalian cells
table: antibiotics conc.
Blasticidin 10μg/mL
G418 (neomycin) 1000ug/mL
Hygromycin 100μg/mL
Zeocin (bleomycin) 100μg/mL
Puromycin 1μg/mL
xgpt 12.5μg/mL
Puromycin
Stock concentration 1 mg/mL in DDW
Working concentration 0.5-3 ug/mL (usually 1-2 ug/mL)
1-2 days to start killing mammalian cells.
Blasticidin S
Stock concentration 10 mg/mL in DDW
Working concentration 1-30 ug/mL (usually 10 ug/mL)
2-3 days to start killing mammalian cells.
Zeocin (bleomycin)
Stock concentration 100 mg/mL in DDW
Working concentration 0.5-1000 ug/mL (usually 100 ug/mL)
4-5 days to start killing mammalian cells.
Expensive.
Hygromycin
Stock concentration 50-100 mg/mL in DDW
Working concentration 100-1000 ug/mL (usually 100-200 ug/mL)
4-5 days to start killing mammalian cells.
G418 (Neomycin)
Stock concentration 50-100 mg/mL in DDW (acidic)
Working concentration 100-1000 ug/mL (usually 700-1000 ug/mL)
6-7 days to start killing mammalian cells.
Niwa and his colleagues examined the relation between antibiotics concentration and expression level. This is very nice paper!
Kinetics of drug selection systems in mouse embryonic stem cells.
Yuhki Nakatake, Setsuko Fujii, Shinji Masui, Toshimi Sugimoto, Satomi Torikai-Nishikawa, Kenjiro Adachi and Hitoshi Niwa
BMC Biotechnology 201313:64
Antibiotics for mammalian cells
Date : 2018-03-16
Update Date :
Author : gmaryu.icon