Antibiotics for mammalian cells

table: antibiotics conc.

Blasticidin 10 μg/mL

G418 (neomycin) 1000 μg/mL

Hygromycin 100 μg/mL

Zeocin (bleomycin) 100 μg/mL

Puromycin 1 μg/mL

xgpt 12.5 μg/mL

Puromycin

Stock concentration 1 mg/mL or 10 mg/mL in DDW

Working concentration 0.5-3 μg/mL (usually 1-2 μg/mL)

1-2 days to start killing mammalian cells.

Blasticidin S

Stock concentration 10 mg/mL in DDW

Working concentration 1-30 μg/mL (usually 10 μg/mL)

2-3 days to start killing mammalian cells.

Zeocin (bleomycin)

Stock concentration 100 mg/mL in DDW

Working concentration 0.5-1000 μg/mL (usually 100 μg/mL)

4-5 days to start killing mammalian cells.

Expensive.

Hygromycin

Stock concentration 50-100 mg/mL in DDW

Working concentration 100-1000 μg/mL (usually 100-200 μg/mL)

4-5 days to start killing mammalian cells.

G418 (Neomycin)

Stock concentration 50-100 mg/mL in DDW (acidic)

Working concentration 100-1000 μg/mL (usually 700-1000 μg/mL)

6-7 days to start killing mammalian cells.

Niwa and his colleagues examined the relation between antibiotics concentration and expression level. This is very nice paper!

Kinetics of drug selection systems in mouse embryonic stem cells.

Yuhki Nakatake, Setsuko Fujii, Shinji Masui, Toshimi Sugimoto, Satomi Torikai-Nishikawa, Kenjiro Adachi and Hitoshi Niwa

BMC Biotechnology 201313:64

Antibiotics for mammalian cells

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