分裂酵母　培地 　Fission yeast medium recipe

YEA (1 L)

Yeast extract 10 g

Glucose 20 g

Adenine 225 mg

(Agar 20 g for plate)

Distilled water 1000 mL

EMM (1 L)

Potassium hydrogen phthallate 3 g

Na2HPO4 2.2 g

x50 Salts 20 mL

x1000 Vitamins 1 mL

x10000 Minerals 100 μL

(Agar 20 g for plate)

DW 820 mL

After autoclave, add supplements and fill up by DW (followed by filter sterilization).

20% Glucose (x10)

25% NH4Cl (x50)

Nitrogen source powders, final 20 mM Glutamate, Proline, or Phenyle Alanine

SPA (1 L)

Glucose 10 g

KH2PO4 1 g

x1000 Vitamins 1 mL

Agar 20 g

DW 1 L

Antibiotics for pombe

table: antibiotics for pombe

Antibiotics Stock conc. working conc. Resistant gene

G418 100 mg/mL 0.1 mg/mL kanMX6

Hygromycin B 300 mg/mL 0.3 mg/mL hyg

ClonNat 100 mg/mL 0.1 mg/mL nat

Blasticidin 100 mg/mL 0.1 mg/mL bsd